Abstracto

Preparation and Standardisation of Poly-Nutraceuticals Formulation from Seeds and Comparison of its In Vivo AntiObesity Activity with the Marketed Product

Manu N, Sujani Kamble

Background: Obesity-associated is rapidly increasing throughout the world. It is generally recognized that natural products with a long history of safety can modulate obesity.

Aim: To investigate the development of obesity in response to High fat cafeteria diet and to estimate the effect of prepared poly-nutraceutical formulation from seeds on bodyweight, anthropometric parameters, organ and fat pad weights, serum lipid profiles, hepatic assay in wistar rats.

Method: Seeds were collected & authenticated from authorised centres. Preliminary Pharmacognostical evaluation parameters of seeds and their powders like LOD, microscopy of seeds powder were carried out. Evaluation of their standardisation parameters like Bulk density, tapped density, Carr’s (compressibility)index, Hauser’s ratio, angle of repose were carried out which were finally used in preparation of nutraceutical powder formulation.

Experimental study was carried out using adult Female Wistar rats weighing between 150-170 g. Obesity was induced by feeding with an high fat cafeteria diet for over a period of 40 days. Animals were divided into 5 groups, 6 rats in each group: Group 01: Negative Control (fed with normal pellets chows), Group 02: Positive control group (fed with normal pellets 50%+HFCD 50%), Group 03: Pellet chew, Cafeteria diet(HFCD)+High dose of prepared formulation (400 mg), Group 04: Pellet chew, HFCD+Low dose of prepared formulation (200 mg), Group 05: Normal chew pellets+HFCD +marketed formulation (300 mg) as Standard, The prepared poly-nutraceutical formulation powder and marketed formulation was administered orally in 3% acacia suspension with the aid of oral gavage every day for about 6 weeks (40 days), 400 mg of diet is high dose, 200 mg of diet is low dose of prepared formulation and 300 mg of marketed formulation powder as standard. Pre and Post analysis of Body weight, anthropometric parameters, organ and fat pad weights, lipid profile, serum biochemical analysis liver homogenation (LPO assays), liver histopathology of experimental animals were carried out.

Results: Data showed that feeding with cafeteria diet significantly increased final body weight, fat pad weights, glucose, Triglycerides (TG), LDL, total cholesterol levels in serum, concentration compared with controls, meanwhile treatment with prepared poly-nutraceutical formulation significantly normalized the body weight, anthropometric parameters, glucose and lipid profile. Serum ALT, AST were significantly higher in a Cafeteria diet group compared with normal controls; and administration of prepared poly-nutraceutical formulation significantly lessened the effect of the Cafeteria diet.

Anthropometric parameters were found to be increased in a Cafeteria diet group in comparison with the control group. The treatment with prepared poly-nutraceutical formulation normalized the condition.

Conclusion: Cafeteria diet induced obesity associated with a disturbed lipid profile (TGs, TC, LDL, HDL), liver (AST, ALT), this may have implications for the progress of obesity related problems. Treatment with prepared poly-nutraceutical formulation of flax, cucumber, chia, black seeds improved obesity and its associated metabolic problems in different degrees. Moreover poly-nutraceutical formulation of flax, cucumber, chia, black seeds might be a safe nutraceutical formulation on the organs whose functions were examined, as a way to surmount the obesity state; and it has a distinct anti-obesity effect.