Abstracto

Evaluation of the effect of geographical and climatic conditions on the chemical composition of a plant drug by HPTLC

Saifi Alimuddin and Patel NM

High performance thin layer chromatography (HPTLC) is the simplest separation technique available today which provides higher precision and accuracy with extreme flexibility for the various steps (stationary phase, mobile phase, development technique and detection). We planned our work to investigate the HPTLC fingerprint profile of alcoholic extracts of stem bark of F. bengalensis Lin. collected from three different geographical regions namely Sangam Vihar (Delhi), Modasa (Gujarat) and Ramnagar (Uttaranchal) and the HPTLC spectra of all the drug extracts were compared to assess the effects of geography and climatic conditions on the chemical composition of this drug. The code name given for drugs from Delhi, Gujarat and Uttaranchal are FB/DL, FB/GJ and FB/UA respectively. We found that all the extracts showed the presence of different compounds as the geography and environmental conditions changed.