Abstracto

Isolation, Screening and Identification of Terpene Resistant Microorganisms from Decayed Yellow Orange Citrus Fruits.

Deepthi Priya K, Manish Petkar, and Ghanta Venkaiah Chowdary

Novel terpene resistant microbial isolates were obtained from decayed yellow orange citrus fruits (Citrus reticulata) collected from distant locations in Navi Mumbai fruit market. A total of nine microorganisms were isolated and coded as 24B1, 48B1, 48Y1, 48Y2, 48Y3, 120Y1, 120Y2, 2WY1 and 2WASP. Terpene resistant microbial isolates were screened by checking their growth in increasing concentrations of monoterpenes, limonene and alpha pinene. Five strains, 24B1, 48Y2, 48Y3, 2WY1 and 2WASP were selected by their resistance to terpenes in yeast malt broth in primary screening. Subsequently, three strains, 24B1, 48Y2 and 2WASP were selected upon their capacity of using these terpenes as sole carbon source in mineral media in secondary screening .Hence the above three isolates may be further processed to retain their activity in degrading terpenes to produce valuable aromatic chemicals.