Abstracto

Screening of Alkalophilic and Thermophilic Potential Isolate For Production of Polygalacturonase

M.P.Kusuma , D.Sri Rami Reddy, Mukund Sharma

Pectinases are an enzyme group that catalyzes pectin substance degradation through depolymerization (hydrolases and lyases) and deesterification (esterases) reactions. One of the most important and widely used commercial pectinase is polygalacturonase. Pectinolytic enzymes are naturally produced by many organisms like bacteria, fungi, yeasts, nematodes, protozoan and plants. Alkaline pectinases are generally produced by bacteria particularly species of Bacillus, but are also made by some filamentous fungi and yeasts (1) and acidic pectinases are produced by fungi. A total of 66 bacterial colonies were isolated from six sampling sites. Out of 66 isolates 8 isolates showed polygalacturonase actity and C4 isolate showed maximum actity. The potential new isolate on polygalacturonase production was tested against three polygalacturonase producing microorganism. Comparately isolate C4 showed maximum enzyme actity hence it was chosen for in depth study. The isolate C4 was characterized and identified as Bacillus subtilis C4 by biochemical studies and 16 rDNA analysis. The new Bacillus subtilis was used for polygalacturonase production under submerged ferementation. Growth profile of the above isolate was studied. The results revealed that the organism was showing maximum log phase till 24hr period after which the growth gradually reduced and remained stable till 72h indicating stationary phase at 400C and pH 11.0