Abstracto

Isozyme Electrophoresis and Morphometric Comparison of Reed (Imperata cylindrical) Adaptation to different Altitudes

HailuWeldekirosHailu,DanangHariKristiyanto, Ali Ramadan Ali ALatawi,SalehMuhammedRaqib

Reed (Imperatacylindrical) is tall woody perennial grass with hollow slender stems and sharp leaves. In Indonesia it is traditional medicinal plant used as treatment for lowering high blood pressure and pain reliever.The objectives of this research were to compare morphometric differences based on altitude adaptations among reeds (Imperata cylindrical) and to analyze adaptation of reeds (Imperata cylindrical) to different altitudes based on the Isozyme electrophoresis patterns. For the morphometric studyheight, wet and dry body mass, dry root to shoot mas ratio were measured. Additionally, Peroxidase Isozyme electrophoresis pattern was studied. The result showed thatthe valuesof altitudes1905m>1500m>1000m>500m for all morphometric parameters.Based on morphometric measurements it was found that reeds prefer higher altitudes for their development. Although Isozymes are powerful molecular tools for gene variability within and between populations of plants, they are independent of altitudinal differences. Generally, biological markers such as morphology, biochemical and molecular genetic markers are useful biotechnology tools that can be used to study plant taxonomy and adaptation.