Abstracto

In Vitro Shoot Regeneration of Corchorus acutangulus Lam. (Tiliaceae).

K Padmavathy, S Paulsamy, and J Thambiraj

The herb, Corchorus acutangulus is an important medicinal plant belongs to the family Tiliaceae. The present study describes the efficient in vitro regeneration protocol standardized for this species through leaf explant. The leaf explants were cultured on Murashige & Skoog (MS) medium supplemented with 2,4-D (1.5 mgl-1) and NAA (0.2 mgl-1) for efficient callus induction (84.43%). Multiple shoots (83%) were developed in the MS medium fortified with IBA (2.0 mgl-1) and TDZ (0.2 mgl-1). Better rooting response (74%) was observed on half strength MS medium containing IBA (2.0 mgl-1) and Kn (0.2 mgl-1). Regenerated plantlets were successfully acclimatized and hardened off inside the culture room and then transferred to green house with 81% survival rate.