Abstracto

Development of Alternate Model for Phototoxicity Assessment of Drugs by using Bacterial strains

Gaurav Sadhwani, Abhay Kumar Tiwari, Shweta Menghani, Md. Anas Siddiqui, R. K Hans

In this study the phototoxicity of drugs Ofloxacin, Doxycycline and Torsemide was determined using two bacterial strains S.epidermidis & E.coli (Dh5-α). It was found that Staphylococcus epidermidis was resistant against reactive oxygen species generated by drugs on exposure to UV-A while E.coli strain Dh5-α was affected by the phototoxic effect of these drugs which was shown by the increase in inhibition zones after exposing them under UV-B radiation. Thus, the study showed that E.coli can be used as a reference to determine the phototoxic effect of drugs whereas S.epidermidis cannot be used for this purpose. The study also suggests the phototoxic potential of drugs, Ofloxacin, Doxycyclin and Torsemide, in presence of UV radiation (especially UV-B fraction). Among nine selected drugs only above three drugs had their absorption maxima in UV-B region. After analysis it produce a phototoxic effect on exposure to UV-B radiation.